Identifying Mixed Mycobacterium tuberculosis Infection and Laboratory Cross-Contamination during Mycobacterial Sequencing Programs
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چکیده
منابع مشابه
Mycobacterial cross contamination during radiometric culturing.
Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was demonstrated.
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A diagnosis of tuberculosis (TB) is rarely disputed if Mycobacterium tuberculosis is isolated from a clinical specimen; however, specimen contamination may occur (1-3). Identification of TB strain patterns through molecular typing or DNA fingerprinting is a recent advancement in TB laboratory techniques (3-7). CDC's National Tuberculosis Genotyping and Surveillance Network (NTGSN) performs DNA ...
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Objective: To investigate possible pseudo-outbreak of Mycobacterium tuberculosis (MTB) during a two week period July 2-15th 2005, as suggested by an increase in incidence of newly diagnosed patients with tuberculosis. Many of them had negative – smears for AFB and only one positive-MTB culture. Methodology: Retrorespective surveys of all medical and laboratory data using standard epidemiologica...
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A prospective study of false-positive cultures of Mycobacterium tuberculosis that resulted from laboratory cross-contamination was conducted at three laboratories in California. Laboratory cross-contamination accounted for 2% of the positive cultures. Cross-contamination should be a concern when an isolate matches the genotype of another sample processed during the same period.
متن کاملExtensive cross-contamination of specimens with Mycobacterium tuberculosis in a reference laboratory.
A striking increase in the numbers of cultures positive for Mycobacterium tuberculosis was noticed in a mycobacterial reference laboratory in Campinas, Sao Paulo State, Brazil, in May 1995. A contaminated bronchoscope was the suspected cause of the increase. All 91 M. tuberculosis isolates grown from samples from patients between 8 May and 18 July 1995 were characterized by spoligotyping and IS...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2018
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00923-18